Abstract
Background: 6-Shogaol is one of the major bioactive compounds found in ginger (Zingiber officinale) and has wide-spectrum pharmacological activities such as antioxidant, anti-inflammatory, antimicrobial & antidiabetic. Nevertheless, its clinical applications are limited by poor stability, low water solubility, and low bioavailability. Nanotechnology-based drug delivery systems are an attractive approach for enhancing the stability, controlled release, and therapeutic performance of bioactive compounds. Due to their biocompatibility, high surface area, and excellent promotion effect on drug delivery, zinc oxide (ZnO) nanoparticles are potential carriers for drug-induced treatment. Consequently, 6-shogaol-loaded ZnO may have better release properties and antioxidant activity, making it an applicable way to improve the treatment of some oxidative stress chronic diseases with the low therapeutic effect of 6-shogaol. Objective: This study was conducted to investigate the preparation of 6-Shogaol in the form of nanoparticles loaded in zinc oxide and to test the release of 6-Shogaol from the layer of zinc oxide and examine the efficiency of 6-Shogaol against oxidative stress. Methods: Free and nanoparticle forms of 6-Shogaol and zinc oxide were examined by different tests such as (FTIR, XRD, SEM & AFM), also testing the release of 6-Shogaol nanoparticles from layers of zinc oxide in different solutions, testing of chemical elements and examination of antioxidants such as MDA, SOD, GSH, and CAT levels. Results: the results show successful intercalation of 6-Shogaol between layers of zinc oxide through the test of FTIR and XRD because have additional peaks and the XRD value near to two theta, also the surface of zinc oxide and 6-Shogaol was examined AFM and SEM were used to detect changes in the surface of the intercalated 6-Shogaol by zinc oxide; the intercalation of 6-Shogaol improved its release to base compared to acid and neutral buffers. For MDA and SOD concentrations in Group G3 (STZ-treated), MDA concentrations were significantly increased, and SOD concentrations significantly decreased. In contrast, SOD, CAT, and GSH concentrations in Groups G4 and G5 (6-Shogaol nanoparticle-treated) were significantly increased compared to controls, while MDA concentrations were lower. Conclusion: Reached is that 6-shogaol is an effective drug that will help treat many different types of illnesses as an Antibiotic and an Antioxidant that it is extremely non-toxic to the body. Because 6-shogaol is found only in very small particles in nanometers, it can pass through a cell's membrane and exert its positive effects quite well.
